The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. 2. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. Please refer to the Obsolete Microplate Reader Models for more information. Microplate Readers. Here, we demonstrate performance of the SpectraMax® iD3 Multi-Mode Microplate Reader for assays measuring intrinsic tryptophan fluorescence. #6590-0131) Fluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. Tecan Infinite Microplate Reader. Lysozyme contains two tryptophan residues that. The University of Rouen in Normandy uses the SpectraMax iD3 and. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). Microplate Readers BioTek Epoch 2 Microplate Reader. ” Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. Unparalleled performance on a personalized platform. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Example: [Drawer]. Wide range of applications. , April 14, 2022 – Molecular Devices, LLC. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. 5 87. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Assays can be better optimized by scanning across. Microplate reader settings. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. Step 2: Launch SoftMax Pro Software. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. HTRF-compatible SpectraMax Multi-Mode Readers. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Cuvette Microplate wells. Chapter 1: Introduction The SpectraMax® Mini Multi-Mode Microplate Reader is a multi-detection instrument with a 6- well to 384-well plate read capability. The cuvette must be in place when you read the plate. 100 fmol 384-well (75 µL) 0. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. 1. The system uses two scanning monochromators to determine the optimal excitation and emission settings. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Figure 1. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. The plate reader optics are tunable for absorbance. Choose from a three- (M3), four- (M4), orIn this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Detailed specifications of each model reader are reported in Section 7, “Specifications”. . These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 315 can't find zero order. The SpectraMax® ABS Plus Microplate Reader can run . San Jose, Calif. The Spectramax 250s xenon flash lamp and grating monochromator facilitates direct UV measurement of DNA, RNA, and protein in microplates. Avoid losing high signal. 300 µL of Detergent Compatible Bradford reagent was added to each well. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax i3x. Transcreener ® ADP. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. Published Dec 16, 2020 | Updated May 19, 2023. The advanced optical and. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). 0 out of 5 $ 11,999. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. Highly customizable low volume. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Lower limits of detection for ATP determined using the ATPliteAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. The Quant-iT PicoGreen dsDNA Assay Kit, when run on a SpectraMax microplate reader with SoftMax Pro Software, is a quick, sensitive detection method for double-stranded DNA. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Detection modalities include absorbance (UV-Vis Abs) and. Molecular Devices Spectramax iD3 Microplate Reader. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. 0 out of 5 $ 2,999-20%. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. 6% for the on-board optics (monochromators). A microplate reader as The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Blank subtraction and standard curve fitting were done automatically by the software. The SpectraMax microplate readers can be used to accurately quantify ROS levels using a luminescence-based assay. Download PDF versions of the SpectraMax Microplate Reader User Guides. The screw remains inside the instrument. Page 28 Chapter 5: Read Modes and Read Types 1. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking andThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expandthe boundaries of your research capabilities. The SpectraMax Mini Multi-Mode Microplate Reader performs your favorite applications including ELISA, DNA and protein quantitation, cell viability, gene expression with a user-friendly reader. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 306 plate air cal. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Step 4: Incubate at room temperature for 1 hour, protected from light. This unlocks the transport slide. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. Multimode Microplate Readers. . SpectraMax ® M2 Multi-Mode Microplate Reader. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. 314 low lamp level warning. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. Click SoftMax Pro Software User Guide. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. 20 μL of Component A was added to each well of the microplate. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Bookings are free, but you will need to use iLab to book. Microplate Readers Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Or scan up to 384 wells at any time to determine the best assay conditions. 470 nm). Data exemplifying linear ranges from the three different kits are presented in Figure 2. SpectraMax Microplate Readers. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. SAN JOSE, CA — Molecular Devices, LLC. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. Compact designs. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Table 2 summarizes the results for the uniformity test. Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . 76358-624. The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. 997 (n = 3). The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Add to Cart. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 0 out of 5 $ 5,999. Italic and boldface type are used for emphasis. Category 2 and 3 microplate readers use gradient heating as well. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The eight-channel system consists of 8 sample beams and 8 reference beams, delivering both superior precision and speed of reading across the microplate. It can run samples based on pre-defined. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. SpectraMax M5 reader settings are shown. The instrument uses a paired monochromator and xenon flash. . Molecular Devices SpectraMax M2 Multimode Plate Reader. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The dual monochromator optics allow the. 0 CART STOREVersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Download PDF. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Assays vary depending on. It also integrates with the. With optimized reagents, option to operate the. The Gemini EM can sample a variety of formats, from 6- to 384-well. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. Figure 1. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Plate chamber design of the SpectraMax category 1 microplate readers. Type:Spectrophotometer. 2. See SpectraMax® Microplate Reader User Manual Download Page. IMAP FP Settings in SoftMax ProSpectraMax M5 Multi-Mode Microplate Reader. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate 312 gain calibration failed. SpectraMax iD5 Multi-Mode Microplate Reader. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Cell Cytotoxicity assay procedure. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Materials. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Order Now. For readers without a cuvette port, these options are not shown in the software. begin reading microplates with seven mouse clicks. Selection of Water Constant in PathCheck settings. 40266 Email: drugdiscoverytech@lifetech. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. We would like to show you a description here but the site won’t allow us. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Other technologies such as imaging. The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Offer Ends In: 18 Days 19: 38: 21. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. Molecular Devices. Win7 Manual Spectramax-L or StakMax Driver Installation after Initial Install. Narrow 2nm bandwidths deliver increased measurement accuracy and linearity over the widest range. begin reading microplates with seven mouse clicks. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path. 0200-6263. 303 unable to cal dark current. Add to Cart. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. 2 software: see SoftMax® Pro 7. The advanced optical and. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. keeping with best practices, reader performance should. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. With optimized reagents, option to operate the. SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax 190 reader can be integrated with Molecular . When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 µL sample volume in a. Request a Quote!Introduction. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. In addition, the SpectraMax iD5Next generation SpectraMax® absorbance microplate readers. Molecular Devices. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. 380 nm/Exc. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile microplate reader capable of quantitating samples in a variety of microplate formats. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Microplate reader assays requiring infrared (IR) or red-shifted. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. If it looks fine, then proceed. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. Find regional support. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both superior. Multimode Microplate Reader. Measure more accurately. on the SpectraMax iD3 microplate reader Introduction The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. flexibility of monochromator-based systems. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Or scan up to 384 wells at any time to determine the best assay conditions. 7°C (gradient heating design). Step 2: Launch SoftMax Pro Software. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. 1. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Instrument settings used with the SpectraMax M5 Microplate Reader. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. both cuvette-based and microplate reader applications on the same instrument. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. Our specialization servicing. 230-1000 nm, Monochromator, ABS, FLR (top and bottom) and LUM, with incubation. microplate reader. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Italic and boldface type are used for emphasis. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. For readers without a cuvette port, these options are not shown in the software. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. limit of detection (LLD) for ATP was estimated for each microplate reader using the . BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. For a higher . 000 Euro/Dollars for a high-end multi-mode microplate reader with several detection modes and the best technology for highest sensitivity and fastest detection. 2. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Absorbance (optical density, OD) was read at 570 nm and 605. ATP standard curves read on SpectraMax microplate readers. We would like to show you a description here but the site won’t allow us. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Cell Cytotoxicity assay procedure. Devices’ StakMax® Microplate Stacker in a matter of minutes and . We would like to show you a description here but the site won’t allow us. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Summary of Contents for Molecular Devices SpectraMax M2. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance values to a 1-cm pathlength so that they are the same as values obtained in a 1-cm cuvette. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Add to Cart. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. It was also read on the monochromator-based. both cuvette-based and microplate reader applications Microplate Readers. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. All . SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. Dual-Glo luciferase assay system (Promega Cat. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. 315 can't find zero order. The SpectraMax® i3 is Molecular Devices latest multi-mode microplate reader which delivers several applications including; Ultra-Violet to visible absorbance detection, fluorescence intensity and multi-colour luminescence. MPN:SpectraMax Plus. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. SpectraMax Quant dsDNA Assay Kits are highly selective for dsDNA. Cell Analysis. Note that this procedure is only for the SpectraMax L model. 0 out of 5 $ 11,999. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax® ABS Plus Microplate Reader can run . no. Or, with the reader specifications noted from the Instrument Description section of your reader's. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow toFlexStation® 3 Multi-Mode Microplate Reader. Fill a clean plate with water. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. This unlocks the transport slide. Published Dec 16, 2020 | Updated Dec 16, 2020. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. . Products. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Compact and tunable absorbance microplate readers DATA SHEET. ¨0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingThe SpectraMax iD5 Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Figure 1: Endotoxin standard curves on SpectraMax microplate readers, plotted using a log-log curve fit. 030 fmol Fluorescence Polarization (FP). The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. FI assay. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Reader modes are customized to your specific applications with options to upgrade easily. Detailed specifications of each model reader are reported in Section. In addition, the SpectraMax iD5The SpectraMax® L Luminescence Microplate Reader is a high-performance microplate reader that measures luminescence in 96 and 384-well microplates. Absorbance (optical density, OD) was read at 570 nm and 605. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. The plate reader can measure multiple readouts, including UV. 7°C and the bottom to 36. 7°C (gradient heating design). 1. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays.